Before cataract surgery on a patient with a corneal dystrophy, a technician takes specular photographs so that the endothelial cells can be counted. The endothelium is the innermost layer of corneal cells. The surgeon explains that a low count matters because of what these cells do. What is the job of the corneal endothelial cells?
The endothelial cells pump fluid to keep the cornea clear. They act as a pump that controls how much fluid sits in the corneal layers. Clear layers let light pass and focus properly. When too many cells are lost, fluid builds up. The cornea then swells and turns cloudy, and vision drops. That is why the surgeon wants a cell count before surgery.
The number of endothelial cells is an indicator of corneal health. Cells that are too few, enlarged, or irregular can point to disease. They also warn that the cornea may not withstand the stress of eye surgery. Specular microscopy photographs these cells at great magnification so they can be counted. A slit-lamp attachment can take still photographs, or a video camera can display the cells on a screen. An image from that screen is saved in the medical record. The count helps the surgeon build a treatment plan. A very low count may push the surgeon to change technique or to warn the patient that the cornea may cloud after surgery.
Remember the endothelium as the cornea's water pump. Lose the pump and the cornea clouds, so a count taken before surgery can change the surgical plan. Handle the slit lamp gently and take a sharp, well-focused image, because blurred cells cannot be counted. Knowing what specular microscopy counts, and why, belongs to the biometry content area of the COA exam.
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